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Type and details of transgene |
Is the transgene Plasmodium derived |
Transgene: not Plasmodium |
Transgene name | mCherry |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |

GGCCGCTATTATTGTTCTGTACTTCTTTTGTGAATAGTATTTTTACATATGTACTGTTAA
TTTTAAAATGTGGAATGCCACGAACATATTTTTTCGAACAAAATATATATTTTTTGTGTC
GAAATACATAAATTTGTGTACCTCCTTTTTGTTGTATATATGAAAAAGGAATAAAGTGAA
TGGAGTCGAATTACTCTAGACCCTACAACTTGTATATCACTACTTTATAATACTATAATA
TTATTATTAACATTAAACTATAGAAGAAATATCAACAATACTTATGTATTTGTTCAAACT
TTATCAGTATAGTTTGGTAATCGCATATCATATATTTGTTATGTGTAATATTTTATGAAT
ATGCGTCTTTTTTTTTAACTATAAATAATTCAAAATAAAATAACATAATTAACATTTGCA
AGCATAATTAAGATGATTACTTTCACATTTCATCCAAAGTGGAAAATGACCACCACATTT
GATCGAAGCTTTTACCACTTTTGATGCATTAGCTCTGAAATGTATACAAGCTTTCATTTG
TTAATGTTACAAAGGGAAGTTTTTATTTGGGGGCACATTAAAAGTGAAAAAATTAAAAGT
TAAAAAAGTTAAAAAATGATCGGTGTAGTTGGTCATATAGACATGTTGTTATGCAAGTCT
TTCAAAACGTTCCTCATAAAATAAAAGGAGGGATCTAGAGAGCTTTCAATTCTCCAAACG
GTATTATTGGTATTTTCCGGTACCGAGCTCGAATTCTCTTGAGCCCGTTAATGAAATAGA
TACAATTCATTCATGTTATATACATCTAGAACATAATCTGAATATGGTTCAAGTTAAATG
TCCAAAAATTATAAAAAGTGATGATATTTTTGATGGTAATACCATAATAGACACCAAGGT
AACATCACGAAGTAGTCAACAAAATAATTTTTATTTAGAAAATACAGATGTTGAACCAGA
AGAAATAGAGAAATATAAAAATATAGAATACATACCAGAAAACGATGAAGTAATGCATCT
AGACAAAAAAGAAAAGCTAGATGATATATTACCAGGTGTTATCATATTAGATAAAAATAA
AATGTTCAAAGAAAAAGGACATTTCACTTTTGTTACTCCATTAATTGTAGAAAAGGTATT
AATATTAAAAATATATTGTGATAATACTAAAACAATAATTAATAATATGAAAGGGAAAAA
AGGTATTACAGTAATAAGGATTTCTCAAAATACAACAAAAAATAAATTTTATGGATGTGA
CTTTTCAGGTAATTCTAAAAAAACATTTTACTATTCCAATGTTTATGATTTAGAAAAAAA
AAATGAGTTTTGTGAAATAGAATTAAAAGAAAATATAGTAGTTAGCTTAAATTGTCCAAC
TGGTAAAATTAATCCAAAAAATTGTTTTAGAAATGTATATATAAAAAGTAATATGAATGA
ACAAACAACCGAAAATATAGAAAATATATTTAACGAAATAAAAGTTATAGATGCAGATTA
TTTTATAAATAATTCATCAACCTTTTTGATGATTTCCAAAATTACAAAAAAAGAGTTTGA
TTTTTATTGTACATGTGAAGATTATAAAACCAAAAATATAGGAACAATATATATTAAAAA
TTATGAATATCTAGATTCAAAACCTAAATATAAAAATAAACAAATTTCCTATATAGATGT
AGTTCCATACCCGCGGGGAAAGGGCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACT
GGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT
GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATG
GCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCA
TATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACC
CGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAC
AAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAC
GCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAA
TGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTT
TATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGC
TTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTC
CCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA
AAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCG
GTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAG
TTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCC
GCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTA
CGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTG
CGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACA
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC
CAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTAT
TAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGG
ATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATA
AATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTA
AGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAA
ATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAG
TTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGG
TGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACT
GAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCG
TAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATC
AAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATA
CTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTA
CATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTC
TTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG
GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTAC
AGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGG
TAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGT
ATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCT
CGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGG
CCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATA
ACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCA
GCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGC
GTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTG
AGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTA
TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACA
GCTATGACCATGATTACGCCAAGCTTCCGCGGGTATATGGTAAAGAACCTACTAACACAA
TAAAATATTTAAATAATGTATTTCCTATAAATAAATTTACAGATTTATTTTTTAATACAA
AAGATATAGATATACCAGAAATAAATGATCAGTTTAAAGGTTTTAAATTCTTTATGACAT
CATTTATAAATCATGGATCATATCCACTAACAATAGAATGTGGTGTAACAAATGGTGGAA
CTAGTTATAAAAGAGCAATTATTTTATTGCATGTTCGAACTGATTTAAAAGATAGACCAG
TTTCATTTTGTGATTTTCGAAAAGGAGAATTATATAATTATTTGAATGCTTATACTGAAG
GGGATGTATGCATAATAATTTCCAAATCAAATACAAGTTTTGGTTTTAGATGCCCAGTAA
ATACAAAAAAAATGCCAAAAAATTGTTTTACGCAAGTATATGAAAAAGGGTATCTAAATG
ACGCCAATAAAATTAATACTAAAAATGTTATTAACTATTCATTTGAAAATCCAGAATATG
CGCTAGCTGGTTYTAATTATACATTAACAAAATCGTATCAATTTGAATGTCATTGTGTAG
ATAAAGAAACAGAACAAATTGTAAAAACGGTTTTAGTCAAATATGTAAATGAAGATGAAA
TATATGATTATAATGATTTTCCAATGGTGAATCACAAACCTATTATTGCACATCCAAATA
AAACACATCAAGCTTGCATGCCGTAATATTTTGTTGGTGAGCTTAAAAACTTTTAATATT
GTTTGTGCAAAATTTTTGCTAAGAAAATAATTTTATGAGTCATATTAGAAGTTCATTGAA
TTTGTTATATAAAATAAATTTTATATTCTGCCTATTTTATGTACAACAAATTGCGGAATT
GTACATAATATAATATGAAAAATATATGGCTTTAAATTATAATTGCCAAATAAAATAGGG
AGGGGCCCCCCCCAAGCCCTCAAAGGGTGCATACTATGTTTATAATATATTATTATATAG
CATTATATTAGCATTATATATATTTGTTATTTTACAATACATTCATTTTGCGTGGGGCAA
TGCATTTGCGCTCTTTTTCAGTTCATATTTTTGCAAATAATTGCACTTTCGGAAATTTGA
AAAAATATAAAACCCATAAAAAGGTGGAATACAAAACATTTTTTGCATTTTCGAAGCATA
TAAATATGTATATATATTATGCATACTATATTATGACTATCCTTGCCAATAAAAAAAAAA
TATTATATAAAGGGGACCCCATATATAGGTATATATAATAGCTGCTTTAACTAAGGGGCT
AATAAATGGCCCCGTATTTTGTTTTTTTTATAGCTAATAATAAAAATAAAAAAATAAAAA
GTACGAACTTTAGTCCCATAAATTAAGAAGTATATATGCTTATAAAAAGGATATCAAATA
AATTAATGTTAACTCAAATAAATTAATTTTCATGCAAAAAAATATTTAAAAAAGAATATA
TATATTAAGAAAAATAATTATAAATATTATTTACATATATATACCATAATATATACCCCC
TTTTGATTATATATATATAATATTATATATTTTTCTTTAGAGAAAAAATAAATAGTGTTA
TTTCCTATTATATATCGTAGTTAATTTTAATAATTTACAATACACTATATTTCTTATTTT
ATATAAAATATATTTATAATTAATTTTTTTATATTATATGTATATATTTTAATTGCATTC
TCCTTTTTTAATAAAAATTTTACCCAATTGTTATAGAGCTTGTTATTATTATATAATAAC
AATATAAGAATATTTTTTATATAACATTAAAAAAAATTAACATATTTTTTATTTTTTTTA
AATATTTATTCCAATTTATTTTTAACGGTCAAATTAAAGAAATATATAGATATATATTTA
TTGCTTAAATTATAAGTATATGTTTTTTTAAAAAATTAAAGCCCAAAACATATTTCTTTG
TATTATATACATTTTTGCATTTTACACTATTTTGCCATAAGCACATTAAATATATATATA
TATGCTTGTAGCAAATTAATTAAATAAAATAATTACCAACATTATTATCCCAATAAATTA
CAATTACAATTAAAAAAAGGGATCCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCAT
CATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT
CGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAA
GGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTA
CGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTT
CCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGT
GACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCAC
CAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTC
CGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCT
GAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGT
GCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGA
CTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGA
CGAGCTGTACAAGTAATCTAGAGCAATTTGC
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | hdhfr/yfcu |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | No |
Selection (negative) procedure | 5-fluorocytosine (5-FC) |
Additional remarks genetic modification | |
Additional remarks selection procedure | This reporter mutant expressing mCherry does not contain a drug-selectable marker.
The mutant has been generated in the reference line GIMOPbANKA (RMgm-687). The GIMO mother line is used for introduction of transgenes into the modified 230p locus through transfection with constructs that target the 230p locus. These constructs insert into the 230p locus (‘gene insertion’), thereby removing the hdhfr::yfcu selectable marker (‘marker out’) from the genome of the mother lines. Transgenic parasites that are marker-free are subsequently selected by applying negative drug selection using 5-FC. This selection procedure is performed in vivo in mice. |
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Other details transgene |
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Promoter |
Gene Model of Parasite |
PBANKA_0711900
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Gene Model P. falciparum ortholog |
PF3D7_0818900
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Gene product | heat shock protein 70 |
Gene product: Alternative name | HSP70 |
Primer information details of the primers used for amplification of the promoter sequence 
Primer information details of the primers used for amplification of the promoter sequence
Sequence Primer 1 | CCGGGGTACCGTAATATTTTGTTGGTGAGC |
Additional information primer 1 | 6254 (Asp718); hsp70 promoter F |
Sequence Primer 2 | gggGGATCCCTTTTTTTAATTGTAATTGTAATTTATTGG |
Additional information primer 2 | 4213 (BamHI); hsp70 promoter R |
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3'-UTR |
Gene Model of Parasite |
PBANKA_0711900
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Gene product | heat shock protein 70 |
Gene product: Alternative name | HSP70 |
Primer information details of the primers used for amplification the 3'-UTR sequences 
Primer information details of the primers used for amplification the 3'-UTR sequences
Sequence Primer 1 | gggACTAGTTATTATTGTTCTGTACTTCTTTTGTG |
Additional information primer 1 | 4214 (SpeI); hsp70 3'UTR F |
Sequence Primer 2 | gggGGTACCGGAAAATACCAATAATACCG |
Additional information primer 2 | 4215 (Asp718); hsp70 3'UTR R |
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Insertion/Replacement locus |
Replacement / Insertion | Replacement locus |
Gene Model of Parasite |
PBANKA_0306000
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Gene product | 6-cysteine protein |
Gene product: Alternative name | 230p |
Primer information details of the primers used for amplification of the target sequences
Primer information details of the primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
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