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Details of the target gene |
Gene Model of Rodent Parasite |
PY17X_0303100
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Gene Model P. falciparum ortholog |
PF3D7_0204700
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Gene product | hexose transporter |
Gene product: Alternative name | hexose transporter 1, HT1; PfHT1, PfHT, PbHT, PbHT1 |
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Details of the genetic modification |
Name of the tag | HA tag |
Details of tagging | C-terminal |
Additional remarks: tagging | a triple HA epitope tag on the C-terminus of the PY00899 protein |
Commercial source of tag-antibodies | |
Type of plasmid/construct | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | not reported |
Promoter of the selectable marker | not reported |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | No information is provided on the DNA primers and construct used for tagging PY00899 |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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