Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of of PBANKA_0519900
Protein (function)
To investigate the proteins that interact with the NTF2 domain-containing protein PBANKA_1019700, protein, immunoprecipitation (IP) using anti-mCherry beads was performed and the proteins bound to PBANKA_1019700 were identified through mass spectrometry (MS) (see RMgm-5525). Cytoplasmic proteins, including ubiquitin-related proteins (ubiquitin carboxylterminal hydrolase, PBANKA_0210600; ubiquitin-like protein, PBANKA_0823000; HECT-type E3 ubiquitin ligase UT, PBANKA_0802300) and a VPS13 domain-containing protein (PBANKA_1359300) were detected through IP-MS of 1019700::mCherry. However, RNA-binding proteins, such as NAB2 and GBP2, were not detected. In addition, the conserved Plasmodium protein PBANKA_0519900 was detected
Phenotype
Analysis of the mutant expressing a C-terminal GFP-tagged version of PBANKA_1019700 showed evidence for localization in the cytoplasm of asexual blood stages.
Analysis of a mutant lacking expression of PBANKA_0519900 (RMgm-2526) showed the following: mice infected with Δ0519900 mutants showed lower levels of parasitaemia than mice infected with control parasites and their survival was prolonged compared to mice infected with control parasites
Additional information
To investigate the proteins that interact with the NTF2 domain-containing protein PBANKA_1019700, protein, immunoprecipitation (IP) using anti-mCherry beads was performed and the proteins bound to PBANKA_1019700 were identified through mass spectrometry (MS) (see RMgm-5525). Cytoplasmic proteins, including ubiquitin-related proteins (ubiquitin carboxylterminal hydrolase, PBANKA_0210600; ubiquitin-like protein, PBANKA_0823000; HECT-type E3 ubiquitin ligase UT, PBANKA_0802300) and a VPS13 domain-containing protein (PBANKA_1359300) were detected through IP-MS of 1019700::mCherry. However, RNA-binding proteins, such as NAB2 and GBP2, were not detected. In addition, the conserved Plasmodium protein PBANKA_0519900 was detected (see RMgm-5526).
Other mutants |