Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene disruption,
Introduction of a transgene
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Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 36003062 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
Not applicable
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Other information parent line | |
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The mutant parasite was generated by |
Name PI/Researcher | Deveci G, Aly ASI |
Name Group/Department | Aly Lab, Beykoz Institute of Life Sciences and Biotechnology |
Name Institute | Bezmialem Vakif University |
City | Istanbul |
Country | Turkey |
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Name of the mutant parasite |
RMgm number | RMgm-5234 |
Principal name | Pbnt4(-) |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Wildtype-like oocyst production. Evidence is presented for a reduced (delayed) egress of sporozoites from the oocysts and a defect in salivary gland invasion. Strongly reduced numbers of salivary gland sporozoites. |
Sporozoite | Evidence is presented for a reduced (delayed) egress of sporozoites from the oocysts and a defect in salivary gland invasion. Strongly reduced numbers of salivary gland sporozoites.
Mice injected with wild-type sporozoites showed a prepatent period of 4 days and 7 days for salivary glands sporozoites and hemolymph sporozoites, respectively, and they did not survive due to high parasitemia beyond days 16 and 18 post sporozoite injection, respectively. However, all mice injected with knockout sporozoites did not develop any blood-stage parasitemia till day 21 post sporozoite injection. |
Liver stage | Mice injected with wild-type sporozoites showed a prepatent period of 4 days and 7 days for salivary glands sporozoites and hemolymph sporozoites, respectively, and they did not survive due to high parasitemia beyond days 16 and 18 post sporozoite injection, respectively. However, all mice injected with knockout sporozoites did not develop any blood-stage parasitemia till day 21 post sporozoite injection. |
Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of NT4 and expresses the reporter mTurquoise2 fluorescent protein under control of the eef1a promoter
Protein (function)
Plasmodium is a purine-auxotrophic parasite that lacks de novo purine synthesis and depends on the host to get the required purines. Parasites contain a special class of protein family called as equilibirative nucleoside transporter (ENT) which are central in the transport of nucleoside from host to parasite. Four members (NT1, NT2, NT3, and NT4) of the NT gene family have been identified in the genome of Plasmodium species, which are known to play important roles in purines acquisition from the host.
Phenotype
Wildtype-like oocyst production. Evidence is presented for a reduced (delayed) egress of sporozoites from the oocysts and a defect in salivary gland invasion. Strongly reduced numbers of salivary gland sporozoites.Mice injected with wild-type sporozoites showed a prepatent period of 4 days and 7 days for salivary glands sporozoites and hemolymph sporozoites, respectively, and they did not survive due to high parasitemia beyond days 16 and 18 post sporozoite injection, respectively. However, all mice injected with knockout sporozoites did not develop any blood-stage parasitemia till day 21 post sporozoite injection.
Additional information
Other mutants
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