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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1457700
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Gene Model P. falciparum ortholog |
PF3D7_1244500
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Gene product | PIMMS57 protein |
Gene product: Alternative name | PSOP26 |
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Details of the genetic modification |
Name of the tag | triple-HA |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | hdhfr/yfcu |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | To tag the endogenous psop26 with a 3 × HA tag, a 769-basepair (bp) fragment [nucleotide (nt) positions 1554–2322 bp] of the psop26 gene was amplified from P. berghei genomic DNA gDNA and ligated into the ApaI and SacII sites of the pL0034 plasmid as the 5′ homologous region (5R). Then, 526 bp of the psop26 3′ untranslated region (UTR) (nt + 1– + 526 bp) was amplified and inserted between the KpnI and EcoRI sites as the 3′ homologous region (3R), to yield the final plasmid pL0034-PSOP26-3 × HA. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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