The gene has been targeted for gene deletion using a construct aimed at integration into the genome by double cross-over homologous recombination

The gene has been targeted for disruption in a 'kinome-wide' study for deletion of genes encoding Plasmodium protein kinases (protein kinase-like proteins).

See the paper for additional information on the analysis of the phenotype.

See RMgm-392 for an independen mutant lacking expression of PBANKA_040940. Phenotype analyses of this mutant showed the following characteristics: Reduced ookinete formation; Reduced numbers of oocyst and of midgut-derived sporozoites; Reduced numbers of salivary gland sporozoites; Salivary gland sporozoites showed normal gliding motility, hepatocyte invasion, liver stage development and infectivity to mice.

Disruption of the P. falciparum ortholog, PFC0485w, has been reported by Agarwal, S et al. (2011; J Cell Biochem). In this paper no information on the phenotype of mutants with a disrupted PFC0485w gene is provided.

Disruption of the P. falciparum ortholog has been attempted (Solyakov et al., 2011, Nat Commun, 2:565). After transfection with a KO vector a strong PCR signal diagnostic for gene disruption was observed in transfected populations indicating that this gene is not essential for asexual proliferation. Cloning will however be required to validate this interpretation for this.