Mutant/mutation
In the mutant the uis4 gene has been replaced by the uis4 gene of P. chabaudi (PCHAS_0501300). This gene is under control of the 3' and 5' UTR regions of uis4 of P. berghei. In addition, the mutant expresses GFP under control of the constitutive eef1a promoter

Protein (function)
UIS4  is expressed throughout liver stage development and localizes to the parasitophorous vacuole. UIS4 has a total length of 220 amino acid residues and can be divided into three domains: an amino-terminal domain of 54 residues including the signal peptide encompassing 25 residues, the trans-membrane region and a positively charged carboxy-terminal domain of 143 residues. Inside an infected hepatocyte, UIS4 is distributed at the PVM and the tubovesicular network (TVN), which forms vesicular protrusions into the host cell cytoplasm.

Phenotype
Phenotype in all life cycle stages not different from wild type P. berghei parasites 

Additional information
From the Abstract:
'Early transcribed membrane proteins (ETRAMPs) form a unique protein family in malaria parasites. These molecules are expressed during Plasmodium intracellular phases and inserted at the parasite parasitophorus vacuole membrane (PVM), which constitutes the host-parasite interface. Upregulated in infectious sporozoites 4 (UIS4) is an essential ETRAMP of liver stages of the murine malaria model parasite Plasmodium berghei.We generated transgenic P. berghei parasites where UIS4 was replaced by Plasmodium falciparum ETRAMP8 or ETRAMP10.3. Both ETRAMPs were expressed in transgenic parasite lines, but liver stage maturation was impaired, indicating that the selected ETRAMPs failed to substitute the function of UIS4. As a control, we included the UIS4 ortholog from the murine parasite Plasmodium chaubaudi. We observed that PcUIS4 successfully restores UIS4 function in P. berghei'

PfETRAMP8, PfETRAMP10.3 and PfETRAMP14.2 are the only members of the P. falciparum ETRAMP family expressed in sporozoites. In contrast to UIS4, PfETRAMP10.3 is also expressed in blood stages and is considerably shorter in amino acid length (Table 1). Previous work demonstrated that PfETRAMP10.3 did not complement the function of UIS4 in P. yoelii parasites. 

Other mutants