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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1205000
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Gene Model P. falciparum ortholog |
PF3D7_1006800
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Gene product | single-strand telomeric DNA-binding protein GBP2, putative |
Gene product: Alternative name | GBP2, G-strand binding protein 2 |
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Details of the genetic modification |
Name of the tag | mCherry |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | hdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | To generate transgenic parasites expressing mCherry-fused GBP2 or mCherry-fused NAB2, the gene-targeting vectors for gbp2 (PBANKA_120500) or nab2 (PBANKA_1122000) were prepared by PCR. The PCR products were annealed to either side of the red fluorescent protein gene (mCherry)-hdhfr expressing cassette and amplified by PCR using gene-specific primers. The gene targeting vectors were introduced into the 3’ flanking regions of ORFs of target genes by double-crossover homologous recombination |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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