| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
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| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 33711786
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Srivastava PN, Mishra S |
| Name Group/Department | Division of Molecular Parasitology and Immunology |
| Name Institute | CSIR-Central Drug Research Institute |
| City | Lucknow |
| Country | India |
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| Name of the mutant parasite |
| RMgm number | RMgm-4985 |
| Principal name | Apn1(mch) |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Expression of mCherry-tagged APN1 in schizonts with mitochondrial location |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Expression of mCherry-tagged APN1 in mature oocysts containing sporozoites |
| Sporozoite | Not different from wild type |
| Liver stage | Not different from wild type |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal 3xHA-mCherry tagged version of APN1
Protein (function)
AP endonucleases are components of DNA repair via the base excision repair (BER) pathway. Plasmodium berghei DNA-(apurinic or apyrimidinic site) lyase 1, putative (Apn1, PBANKA_1347400) is one of the two AP endonucleases found in its genome. The main function of Apn1 is to generate a nick at the 5′-phosphodiester bond in the AP site and generate free 5′-phosphate and 3′-hydroxyl groups.
Phenotype
No phenotype detected in a mutant lacking expression of APN1 (RMgm-4984)
Evidence for localization of mCherry-tagged APN1 in mitochondria of schizonts and oocyst sporozoites
Additional information
Other mutants |
*RMgm-4985
