Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of CPW2

Protein (function)
The protein CPW2 was identified in a GFP-affinity pulldown with high accuracy mass spectrometry using the GFP-tagged ookinete crystalloid protein LAP3 (PBANKA_0204500).
This protein is part of the nine-member ‘CPW-WPC’ domain-containing protein family

Phenotype
No discernible GFP signal in ookinetes

Additional information
From the paper:
Our analysis identified a further two proteins, namely PBANKA_1352500 and 1346300, which were previously reported to be crystalloid-resident by GFP tagging. These two proteins are part of the nine-member ‘CPW-WPC’ domain-containing protein family. Interestingly, an additional four members of this family were identified by our analysis of the LAP3 interactome, namely PBANKA_0943400, 1015400, 1449300 and 1218300. We generated GFP-tagged parasite lines for the latter four CPW-WPC proteins, which revealed that PBANKA_0943400 and 1015400, too, displayed weak GFP signal with a crystalloid-like distribution in ookinetes. Although we could not detect discernible GFP signal in ookinetes of the other two parasite lines, possibly due to low expression levels, our findings indicate that at least several CPW-WPC family members are involved with the crystalloid. 

Other mutants