| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 30290224
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Oda-Yokouchi Y, Ishino T, Tsuboi T |
| Name Group/Department | Division of Malaria Research, Proteo-Science Center |
| Name Institute | Ehime University, Matsuyama |
| City | Ehime |
| Country | Japan |
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| Name of the mutant parasite |
| RMgm number | RMgm-4544 |
| Principal name | ΔPbRON12 |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Not different from wild type |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of RON12
Protein (function)
Rhoptry neck protein 12 (RON12) is found only in Plasmodium and is highly conserved across the genus
Phenotype
No phenotype was detected throughout the complete life cycle that was different from wild type parasites
Additional information
Evidence is presented that RON12 is also expressed in sporozoites and that it is localized to the rhoptry body, rather than to the rhoptry neck as described for its localization pattern in merozoites.
RON12 remains at the apical end in sporozoites shortly after invasion of HepG2 cells, suggesting that RON12 is not secreted prior to invasion, which is like PfRON12 in merozoites. Within sporozoites transforming to the early liver stage, RON12 is localized in the cytoplasm as spots. At the late liver stage, punctate RON12 signal is additionally detected at the marginal region of parasites as well as the central area, but remain within the parasite rather than being secreted to the PV space. The signal intensity of RON12 increases during intra-hepatocytic development, suggesting that newly RON12 production occurs during liver stage parasite maturation.
Other mutants |
*RMgm-4544
