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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1101500
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Gene Model P. falciparum ortholog |
PF3D7_0501700
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Gene product | anaphase-promoting complex subunit 3, putative |
Gene product: Alternative name | APC3 |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | The unsuccessful attempts to disrupt this gene indicate an essential function during asexual blood stage growth/multiplication.
See RMgm-4429 for a 'Promoter swap' mutant: the promoter of APC3 replaced by the promoter of ama1 (PBANKA_0915000)
One essential component that drives the cell cycle, and particularly mitosis, in many eukaryotic systems is the Anaphase Promoting Complex/Cyclosome (APC/C). The APC/C is a multi-subunit E3 ubiquitin ligase that promotes cell-cycle progression by covalently tagging regulators such as securin and cyclin B1 with ubiquitin leading to their proteolysis by the proteasome. The mammalian APC/C has 14 core components, and several key adaptor subunits, including cell division cycle protein 20 (CDC20) and the related CDH1. Intriguingly, only four APC/C components have been identified as coded by the Plasmodium genome: APC10, APC11 and APC3 (a tetratricopeptide repeat [TPR] containing subunit), along with CDC20. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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