RMgmDB - Rodent Malaria genetically modified Parasites
SummaryRMgm-323
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Last modified: 26 September 2009, 20:53
*RMgm-323| Successful modification | The gene/parasite could not be changed/generated by the genetic modification. |
| The following genetic modifications were attempted | Gene disruption |
| Number of attempts to introduce the genetic modification | Unknown |
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 17030505 |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone | P. berghei ANKA 2.34 |
| Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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| Attempts to generate the mutant parasite were performed by | |
| Name PI/Researcher | M. Hirai; H. Matsuoka |
| Name Group/Department | Division of Medical Zoology, Department of Infection and Immunity |
| Name Institute | Jichi Medical University School of Medicine |
| City | Tochigi |
| Country | Japan |
Disrupted: Mutant parasite with a disrupted gene| top of page | |||||||||||||||||||||||||
| Details of the target gene | |||||||||||||||||||||||||
| Gene Model of Rodent Parasite | PBANKA_0910300 | ||||||||||||||||||||||||
| Gene Model P. falciparum ortholog | PF3D7_1138400 | ||||||||||||||||||||||||
| Gene product | guanylyl cyclase | ||||||||||||||||||||||||
| Gene product: Alternative name | Guanylyl cyclase alpha, GCα | ||||||||||||||||||||||||
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| Details of the genetic modification | |||||||||||||||||||||||||
| Inducable system used | No | ||||||||||||||||||||||||
| Additional remarks inducable system | |||||||||||||||||||||||||
| Type of plasmid/construct used | Unknown | ||||||||||||||||||||||||
| PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
| Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
| Plasmid/construct map | |||||||||||||||||||||||||
| Plasmid/construct sequence | |||||||||||||||||||||||||
| Restriction sites to linearize plasmid | |||||||||||||||||||||||||
| Partial or complete disruption of the gene | Unknown | ||||||||||||||||||||||||
| Additional remarks partial/complete disruption | |||||||||||||||||||||||||
| Selectable marker used to select the mutant parasite | Unknown | ||||||||||||||||||||||||
| Promoter of the selectable marker | Unknown | ||||||||||||||||||||||||
| Selection (positive) procedure | Unknown | ||||||||||||||||||||||||
| Selection (negative) procedure | No | ||||||||||||||||||||||||
| Additional remarks genetic modification | In the paper it is reported that repeated attempts were made to disrupt the gene. No information/details is provided on the number of attempts to disrupt the gene, the construct used to disrupt the gene and the sequence of the primers used to amplify the target regions. An independent unsuccessful attempt to disrupt the guanylyl cyclase-α gene is described in RMgm-324. (gene models GCα: PB001219.00.0, PB000256.00.0). Two different genes with high homology to guanylyl cyclases (GCα and GCβ) have been identified in Plasmodium (GCβ, guanylyl cyclase beta; Gene models for GCß PB000752.03.0; PB300849.03.0; PB001059). | ||||||||||||||||||||||||
| Additional remarks selection procedure | |||||||||||||||||||||||||
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Primer information: Primers used for amplification of the target sequences
 Primer information: Primers used for amplification of the target sequences
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