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Type and details of transgene |
Is the transgene Plasmodium derived |
Transgene: not Plasmodium |
Transgene name | GFP (gfp-mu3) |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | gfp (FACS) |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | FACS (flowsorting) |
Selection (negative) procedure | No |
Additional remarks genetic modification | The GFP gene (1 copy) has been inserted into the 230p locus (PB000214.00.0) by double cross-over integration. |
Additional remarks selection procedure | The gfp gene is introduced into the genome using a DNA construct without a drug-selectable marker. This reporter mutant (507cl1; RMgm-7) has been selected by FACS sorting after transfection based on GFP fluorescence. |
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Other details transgene |
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Promoter |
Gene Model of Parasite |
PBANKA_1133300
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Gene Model P. falciparum ortholog |
PF3D7_1357100
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Gene product | elongation factor 1-alpha |
Gene product: Alternative name | eef1a |
Primer information details of the primers used for amplification of the promoter sequence
Primer information details of the primers used for amplification of the promoter sequence
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
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3'-UTR |
Gene Model of Parasite |
PBANKA_0719300
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Gene product | bifunctional dihydrofolate reductase-thymidylate synthase, putative |
Gene product: Alternative name | dhfr/ts |
Primer information details of the primers used for amplification the 3'-UTR sequences
Primer information details of the primers used for amplification the 3'-UTR sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
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Insertion/Replacement locus |
Replacement / Insertion | Replacement locus |
Gene Model of Parasite |
PBANKA_0306000
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Gene product | 6-cysteine protein |
Gene product: Alternative name | 230p |
Primer information details of the primers used for amplification of the target sequences
Primer information details of the primers used for amplification of the target sequences
Sequence Primer 1 | 5'-cccaagcttccgcgggtatatggtaaagaacctactaacac |
Additional information primer 1 | primer L1345: 0.7kb 5'region of PB000214.00.0 (230p gene) |
Sequence Primer 2 | 5'-cccaagcttgatgtgttttatttggatgtgc |
Additional information primer 2 | primer L1346: 0.7kb 5'region of PB000214.00.0 (230p gene) |
Sequence Primer 3 | 5'-ccggaattctcttgagcccgttaatg |
Additional information primer 3 | primer L1347: 1kb 3'region of PB000214.00.0 (230p gene) |
Sequence Primer 4 | 5'-tccccgcgggtatggaactacatctatatagg |
Additional information primer 4 | primer L1348: 1kb 3'region of PB000214.00.0 (230p gene) |
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