top of page |
Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1233600
|
Gene Model P. falciparum ortholog |
PF3D7_0518800
|
Gene product | secreted ookinete protein, putative |
Gene product: Alternative name | PSOP13, putative secreted ookinete protein 13 |
top of page |
Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
|
Type of plasmid/construct used | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
|
Plasmid/construct map |
|
Plasmid/construct sequence |
|
Restriction sites to linearize plasmid |
|
Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
|
Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | Partial gene model. Second gene model involved in the genetic modification: PB300830.00.0 |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | TTGGGCCCAATAACTATCGTTTTTTACAACG |
Additional information primer 1 | AE13a (ApaI); 5' |
Sequence Primer 2 | CCAAGCTTTTGATGCGATACTAAATATTACG |
Additional information primer 2 | AE13b (HindIII); 5' |
Sequence Primer 3 | TGAATTCGTAAGATAAATATTTTGTTGCCT |
Additional information primer 3 | AE13c (EcoRI), 3' |
Sequence Primer 4 | GGGGATCCTTTTATACCATTCCTGAAAGATG |
Additional information primer 4 | AE13d (BamHI), 3' |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
|
top of page |