Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of PBANKA_114540 under the control of the constitutive eef1a promoter (and the 3'-UTR of the calmodulin gene). The gfp-tagged gene is introduced on a circular plasmid.

Protein (function)
The protein was selected based on the presence of a PEXEL motif.

Phenotype
Expression of the GFP-tagged protein in blood stages and is exported into the red blood cell (RBC). Localization in the RBC cytoplasm (punctuate localization)

Additional information
Fom Moreira et al 2016 Plos One:
In the P. berghei genome sequence  3 phist genes (namely, PBANKA_1145400, PBANKA_1229000 and PBANKA_0700800) were identified via BLAST analysis using PHIST domains and pssm profiles as queries. This result differs from the single P. berghei phist gene described in the original annotation. Orthologs of PBANKA_1145400 and PBANKA_1229000 were identified in the genome nucleotide sequence databases for other rodent malaria parasites; namely, P. yoelii (PY00289 and PY01786, respectively) and P. chabaudi (PCAS_1144900 and PCAS_1229700, respectively). Additional phist genes were not identified in rodent malaria parasites. BLAST analyses using the PHIST domain as queries, identify as reciprocal best hits PBANKA_1229000, the P. vivax PHIST protein PvPHIST/CVC-8195 (PVX_093680), and P. falciparum PF3D7_0801000. This indicates possible orthologous (vertically inherited) relationships and is supported by the observed synteny of adjacent genes, including the ookinete-expressed gene warp, together composing a locus which is conserved across the Plasmodium genus. PBANKA_1229000 is internally localized in the P. berghei genome, but the locus appears to be sub-telomeric in species other than rodent malaria parasites.
The P. berghei PHIST proteins PBANKA_1145400 and PBANKA_1229000 possess similar features to P. falciparum PHIST proteins; namely, signal peptides and PEXEL/HT trafficking motifs; and single PHIST domains, which are divergent in aa sequence with respect to each other. Within the single predicted ORF of PBANKA_0700800 we were unable to identify a signal peptide sequence, and there were no attractive upstream ORFs suggestive of an erroneous gene model. We thus propose that PBANKA_0700800 is a pseudogene. The remaining P. berghei phist genes possess the typical 2-exon gene structure, in which the signal peptide is encoded on the first exon and the PEXEL/HT motif is located within the second exon.

Other mutants
See PHIST