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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1222500
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Gene Model P. falciparum ortholog |
PF3D7_0808200
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Gene product | plasmepsin X |
Gene product: Alternative name | plasmepsin X; aspartyl protease |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | C-terminal c-myc tagging of the gene was successful, confirming that these gene loci can be targeted. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | GGGGTACCTTGTCTGTTATACATATACCCTC |
Additional information primer 1 | AE14a (KpnI); 5' |
Sequence Primer 2 | TTGGGCCCTCAGTTTTTATTAACGTTGTTAC |
Additional information primer 2 | AE14b (endogenous HindIII site); 5' |
Sequence Primer 3 | ATGGGAGAGGAATTCATAAATAG |
Additional information primer 3 | AE14c (EcoRI); 3' |
Sequence Primer 4 | GGGGATCCAGTTTTAAATTGCATACGAAAAC |
Additional information primer 4 | AE14d (BamHI); 3' |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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