Additional remarks phenotype | Mutant/mutation
In the 'promoter swap mutant': the promoter of ark2 gene has been replaced with the asexual blood stage promoter clag9 (PBANKA_0836300). This promoter is active in asexual blood stages but not in gametocytes.
In addition it expresses GFP under the constitutive eef1a promoter.
Protein (function)
Aurora kinases (AKs) are a conserved family of spindle-associated protein kinases, with critical roles in four aspects of cell division: (I) driving mitotic/meiotic spindle assembly and disassembly, (II) regulating spindle pole structure and dynamics, (III) promoting accurate chromosome segregation, and (IV) orchestrating cellular fission at cytokinesis. Plasmodium spp. have three divergent aurora-related kinases (ARK1-3) and lack most canonical scaffolds/activators. The parasite uses unconventional modes of chromosome segregation during endomitosis and meiosis in sexual transmission stages within mosquito host. Functional studies with both the human parasite Plasmodium falciparum and rodent parasite Plasmodium berghei have suggested that all three Plasmodium ARKs are likely essential for proliferation in asexual blood stage schizogony.
Phenotype
Normal growth/multiplication of asexual blood stages.
ARK2 transcription was downregulated in Pclag-ark2 gametocytes as shown by qRT-PCR. Neither mitosis in male gamete formation (exflagellation) nor meiosis in zygote differentiation (ookinete development) were affected. Normal formation of mature ookinetes with wild-type-like motility.
A significant reduction (up to 70 %) in the number of oocysts per mosquito midgut, detectable from day 7 post-infection, and remaining significantly lower through to day 21. Microscopic imaging of the midguts revealed that the few oocysts present were smaller than those of wild-type parasites after day 7 and absence of sporozoite formation inside oocysts (some oocysts contained dark granules, and some had a pycnotic appearance). No midgut and salivary gland sporozoites.
Additional information
In the paper evidence is presented for a unique scaffold of an aurora-related kinase (ARK2; PBANKA_0407400; PF3D7_0309200; serine/threonine protein kinase ARK2, putative) at the spindle including the microtubule plus end-binding protein EB1 (PBANKA_0405600; PF3D7_0307300; end-binding protein 1), lacking conserved Aurora scaffold proteins. Gene function studies indicate complementary functions of ARK2 and EB1 in driving endomitotic divisions and thereby parasite transmission.
To examine the role of ARK2 during sexual stages we first tagged the endogenous ARK2 locus with sequence encoding an auxin-inducible degron (AID) and an HA epitope tag to degrade the fusion protein in the presence of auxin in a parasite line expressing the TIR1 protein (Philip and Waters, 2015). Although the genetic modification was confirmed by diagnostic PCR, addition of auxin to gametocytes did not lead to ARK2-AID/HA degradation and there was no detectable phenotype in male gamete formation. Since the AID system was unsuccessful, we used a promoter trap strategy, replacing the ark2 promoter with that of cytoadherence-linked asexual protein (CLAG – PBANKA_1400600), which is not transcribed in gametocytes.
Since ARK2 is expressed in male gametocytes and parasite development is affected after fertilization, we investigated whether the defect is due to inheritance from the male gamete. We performed genetic crosses between Pclag-ark2 parasites and other mutants deficient in production of either male (Δhap2) or female (Δdozi) gametocytes. Crosses between Pclag-ark2 and Δdozi mutants produced some normal-sized oocysts that were able to sporulate, showing a partial rescue of the Pclag-ark2 phenotype. In contrast, crosses between Pclag-ark2 and Δhap2 did not rescue the Pclag-ark2 phenotype. These results reveal that a functional ark2 gene copy from a male gamete is required for subsequent oocyst development.
Analysis of a mutant expressing GFP-tagged ARK2 (RMgm-5321) showed the following:
- ARK2 is expressed in the nucleus throughout the P. berghei life cycle.
ARK2-GFP showed a punctate nuclear pattern with one or two focal points during blood schizogony and sporogony. It was present at a single focal point with an additional more 219 diffuse nuclear location during early stages of male gamete formation and in the early zygote, but in later zygote stages it had a more dynamic location on the spindle and spindle pole. ARK2-GFP was not detected in mature asexual (merozoites and sporozoites) and sexual (male gametes and ookinetes) stages of development
- Spatiotemporal dynamics of ARK2-GFP during male gamete formation demonstrates its association with rapid spindle dynamics.
The location of ARK2 was investigated by indirect immunofluorescence assay (IFA) and its co-localization with microtubules (MTs) in activated gametocytes was analysed by labelling MTs with an α-tubulin antibody. Alpha-tubulin antibody detected both nuclear mitotic spindles and developing cytoplasmic axonemes, but ARK2 colocalized only with the mitotic spindles at all stages of male gamete formation. This result provides evidence that ARK2 is involved in mitosis within the male gametocyte. Deconvolution microscopy confirmed that ARK2 is located on mitotic spindles during male gamete formation.
- ARK2 and kinetochore dynamics are associated, but cytoplasmic axonemal microtubule dynamics are not, male gamete formation.
To investigate further the association of ARK2 and the mitotic spindle during male gametogony, we compared its location with that of the kinetochore marker NDC80 and cytoplasmic axonemal protein kinesin-8B. Parasite lines expressing ARK2-mCherry and NDC80-GFP were crossed, and the progeny were analysed by live-cell imaging to establish the spatiotemporal relationship of the two tagged proteins. The location of both ARK2-mCherry and NDC80-GFP was next to the stained DNA, and with a partial overlap, although NDC80-GFP was always closer to the DNA. Parasite lines expressing ARK2-GFP and kinesin-8B-mCherry were crossed and examined by live cell imaging of both markers. One to two minutes after gametocyte activation, ARK2-GFP was observed close to the DNA and adjacent to, but not overlapping, the kinesin-8B-mCherry tetrad. ARK2-GFP remained distributed on spindles, while there was duplication of kinesin-8B-mCherry labelled tetrads. In later stages of male gametogony, ARK2-GFP remained associated with spindles and spindle poles, while kinesin-8B-mCherry showed a distinct cytoplasmic axonemal location
- Tracing ARK2-GFP location during the zygote to ookinete transition indicates a role at the meiotic spindle.
In zygotes (2h after gametocyte activation and fertilisation), ARK2-GFP was detected at one or two foci. These foci migrated away from each other over the next 8-10h through development into stage IV ookinetes, to opposite sides of the nucleus. During this time, the ARK2-GFP signal appeared to radiate into the centre of the nucleus, typical of a classic metaphase spindle arrangement. These two foci then divided again to form four foci, before the signal faded into a diffuse distribution within nuclei of mature ookinetes. The location of ARK2 relative to that of the kinetochore marker, NDC80, was examined during ookinete development in parasite lines expressing ARK2-mCherry and NDC80-GFP. ARK2-mCherry was located on spindles radiating from the poles and NDC80-GFP was detected along the metaphase plate during stages I to III. By stage IV both ARK2 and NDC80 had accumulated at spindle poles.
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