RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-4128
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1329100; Gene model (P.falciparum): PF3D7_1465700; Gene product: plasmepsin VIII, putative
Transgene
 Transgene not Plasmodium: GFP
Promoter: Gene model: PBANKA_0711900; Gene model (P.falciparum): PF3D7_0818900; Gene product: heat shock protein 70 (HSP70)
3'UTR: Gene model: Not available; Gene product: Not available
Replacement locus: Gene model: PBANKA_1329100; Gene product: plasmepsin VIII
Phenotype Oocyst; Sporozoite; Liver stage;
Last modified: 15 February 2017, 18:15
  *RMgm-4128
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption, Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 28192122
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
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The mutant parasite was generated by
Name PI/ResearcherMastan BS, Mishra S
Name Group/DepartmentDivision of Parasitology
Name InstituteCSIR-Central Drug Research Institute
CityLucknow
CountryIndia
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Name of the mutant parasite
RMgm numberRMgm-4128
Principal namepbpm viii (-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
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Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNormal numbers of oocysts produced. Normal numbers of midgut sporozoites inside oocysts. Reduced numbers of hemolymph sporozoites and no salivary gland sporozoites.
SporozoiteNormal numbers of oocysts produced. Normal numbers of midgut sporozoites inside oocysts. Reduced numbers of hemolymph sporozoites and no salivary gland sporozoites. Reduced egress of sporozoites from oocysts. Sporozoites are immotile inside and outside oocysts.
Liver stageNo infection of hepatocytes in vitro or mice by sporozoites isolated from midguts.
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of plasmepsin VIII

Protein (function)
P. berghei has 7 plasmepsins (aspartic proteases (PM IV-PM X).

Phenotype
Normal numbers of oocysts produced. Normal numbers of midgut sporozoites inside oocysts. Reduced numbers of hemolymph sporozoites and no salivary gland sporozoites. Reduced egress of sporozoites from oocysts. Sporozoites are immotile inside and outside oocysts. No infection of hepatocytes in vitro or mice by sporozoites isolated from midguts.

Additional information
Evidence is presented for normal CSP processing in oocysts

Other mutants

  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1329100
Gene Model P. falciparum ortholog PF3D7_1465700
Gene productplasmepsin VIII, putative
Gene product: Alternative name
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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  Transgene: Mutant parasite expressing a transgene
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Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene nameGFP
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
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Other details transgene
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Promoter
Gene Model of Parasite PBANKA_0711900
Gene Model P. falciparum ortholog PF3D7_0818900
Gene productheat shock protein 70
Gene product: Alternative nameHSP70
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
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3'-UTR
Gene Model of Parasite Not available
Gene productNot available
Gene product: Alternative name
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_1329100
Gene productplasmepsin VIII
Gene product: Alternative name
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren