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Type and details of transgene |
Is the transgene Plasmodium derived |
Transgene: not Plasmodium |
Transgene name | GFP |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct | Plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
SacII/KpnI
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | The wild-type siap-1 locus is targeted with a construct containing the 5´and 3´ UTR of Pbsiap-1, the positive selection marker Tgdhfr-ts, and the gfp coding sequence under control of the csp promoter region. After double cross-over homologous recombination, the siap-1 open reading frame is substituted by the selection marker and the gfp cassette. |
Additional remarks selection procedure | |
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Other details transgene |
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Promoter |
Gene Model of Parasite |
PBANKA_0403200
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Gene Model P. falciparum ortholog |
PF3D7_0304600
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Gene product | circumsporozoite (CS) protein |
Gene product: Alternative name | CSP |
Primer information details of the primers used for amplification of the promoter sequence
Primer information details of the primers used for amplification of the promoter sequence
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
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3'-UTR |
Gene Model of Parasite |
PBANKA_0719300
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Gene product | bifunctional dihydrofolate reductase-thymidylate synthase, putative |
Gene product: Alternative name | dhfr/ts |
Primer information details of the primers used for amplification the 3'-UTR sequences
Primer information details of the primers used for amplification the 3'-UTR sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
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Insertion/Replacement locus |
Replacement / Insertion | Replacement locus |
Gene Model of Parasite |
PBANKA_1006200
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Gene product | sporozoite invasion-associated protein 1 |
Gene product: Alternative name | Sporozoite Invasion-Associated Protein-1; SIAP-1; SIAP-1/ag17/S5 |
Primer information details of the primers used for amplification of the target sequences
Primer information details of the primers used for amplification of the target sequences
Sequence Primer 1 | TCCCCGCGGAGTTAACTCGAAAAAAATAAGGACG |
Additional information primer 1 | SIAP-1_REP1 (SacII) |
Sequence Primer 2 | ATAAGAATGCGGCCGCTTTTAAGATATACCCCTTAATGAGAGC |
Additional information primer 2 | SIAP-1_REP2 (NotI) |
Sequence Primer 3 | CCCAAGCTTGATGTATGTATAATACCGAAAATTGG |
Additional information primer 3 | SIAP-1_REP3 (HindIII) |
Sequence Primer 4 | CGGGGTACCCGCCTTATTTAAGGGATAAAGAATGGG |
Additional information primer 4 | SIAP-1_REP4 (KpnI) |
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