RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1263
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_0941300; Gene model (P.falciparum): PF3D7_1105600; Gene product: translocon component PTEX88
TaggedGene model (rodent): PBANKA_1327300; Gene model (P.falciparum): Not available; Gene product: fam-a protein
Name tag: mCherry
Phenotype Asexual bloodstage;
Last modified: 23 May 2015, 13:03
  *RMgm-1263
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption, Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 25820521
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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The mutant parasite was generated by
Name PI/ResearcherMatz JM; kooij TW
Name Group/DepartmentParasitology Unit
Name InstituteMax Planck Institute for Infection Biology
CityBerlin
CountryGermany
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Name of the mutant parasite
RMgm numberRMgm-1263
Principal namePBANKA_132730-mCherry X ptex88–
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageThe mCherry-tagged protein is exported to the red blood cell cytoplasm
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expressses a C-terminal mCherry-tagged version of PBANKA_132730 and lacks expression of PTEX88, a component of the putative export machinery termed PTEX (Plasmodium Translocon of EXported protein). In addition it expresses GFP under the control of the HSP70 promoter (transgene introduced in the the PTEX88 locus.

This mutant was generated by (mosquito) crossing the mutant expressing the mCherry-tagged mutant with the mutant lacking expression of PTEX88 and expressing GFP (RMgm-942)


Protein (function)

Phenotype
The m-Cherry-tagged protein is exported to the red blood cell cytoplasm

Additional information
The protein was exported into the red blood cell in the absence of PTEX88 (PBANKA_094130), a component of the putative export machinery termed PTEX (Plasmodium Translocon of EXported protein).

Other mutants
See link

  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0941300
Gene Model P. falciparum ortholog PF3D7_1105600
Gene producttranslocon component PTEX88
Gene product: Alternative name
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1327300
Gene Model P. falciparum ortholog Not available
Gene productfam-a protein
Gene product: Alternative name
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Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren