Back to search resultsSummaryRMgm-1156
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene mutation |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 25565321 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Ganter M, Matuschewski K |
Name Group/Department | Parasitology Unit |
Name Institute | F-actin-capping protein subunit alpha, putative |
City | Berlin |
Country | Germany |
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Name of the mutant parasite | |
RMgm number | RMgm-1156 |
Principal name | cpβ(-)::PfCPβ |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Reduced numbers of oocysts. Oocysts produce midgut sporozoites. |
Sporozoite | Reduced numbers of oocysts. Oocysts produce midgut sporozoites. No sporozoites inside salivary glands. |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation See the link F-actin-capping protein subunits for other mutants with mutated F-actin-capping protein subunits Phenotype analyses of the mutant where P. berghei cpβ is replaced with the P. falciparum cpβ gene show a similar phenotype as the mutant lacking P. berghei cpβ. This observation indicates that P. falciparum CPβ cannot complement P. berghei CPβ. A P. berghei mutant in which both the cpα and cpβ genes are replaced with the complete P. falciparum cpα and cpβ genes (RMgm-1157) shows a partial rescue of the phenotype with low numbers of sporozoites present in salivary glands. These observations indicate that a functional CP heterodimer is necessary for normal oocyst and sporozoite development Other mutants |
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Details of the target gene | |||||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_1232400 | ||||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0517600 | ||||||||||||||||||||||||||
Gene product | F-actin-capping protein subunit beta, putative | ||||||||||||||||||||||||||
Gene product: Alternative name | CPbeta, CPβ, UIS17, upregulated in infectious sporozoites gene 17 | ||||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||||
Short description of the mutation | P. berghei cpβ replacement with the complete P. falciparum cpβ gene | ||||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||||
Short description of the conditional mutagenesis | Not available | ||||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||||
Type of plasmid/construct | (Linear) plasmid double cross-over | ||||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | tgdhfr | ||||||||||||||||||||||||||
Promoter of the selectable marker | pbdhfr | ||||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||||
Additional remarks genetic modification | To complement cpβ(-) parasites, we amplified the orthologous P. falciparum CPβ gene using the primers PfCPβ_compforV and PfCPβ_comprevVI and P. falciparum genomic DNA as template. Cloning into the plasmid pPbCPβREP (Ganter et al., 2009) resulted in the plasmid pPfCPβ with PfCPβ under the control of the endogenous PbCPβ promoter and the 3’ untranslated region of PbDHFR/TS. | ||||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||||
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