Summary

RMgm-107
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1123700; Gene model (P.falciparum): PF3D7_0624800; Gene product: conserved Plasmodium protein, unknown function
PhenotypeNo phenotype has been described
Last modified: 18 February 2009, 11:52
  *RMgm-107
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 18974882
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA 507cl1 (RMgm-7)
Other information parent lineP.berghei ANKA 507cl1 (RMgm-7) is a reference ANKA mutant line which expresses GFP under control of a constitutive promoter (PubMed: PMID: 16242190).
The mutant parasite was generated by
Name PI/ResearcherE. Lasonder, C.J. Janse, H.G. Stunnenberg
Name Group/DepartmentDepartment of Molecular Biology
Name InstituteNCMLS, Radboud University Nijmegen
CityNijmegen
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-107
Principal name801; 837
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of PB001101.03.0.

Protein (function)
Unknown. The protein was detected in a proteome analysis of P. falciparum oocysts and sporozoites and was present in salivary-gland sporozoites and not detected in oocyst-derived sporozoites. It contains a signal peptide, a GPI-anchor sequence and a transmembrane region.

Phenotype
The phenotype has not been analysed in detail and the mutants have not been cloned after transfection. Sporozoite production was not significantly different from wild type and sporozoites were infectious to C57BL/6 mice.


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1123700
Gene Model P. falciparum ortholog PF3D7_0624800
Gene productconserved Plasmodium protein, unknown function
Gene product: Alternative name
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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GCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTA
ACCCTCACTAAAGGGAACAAAAGCTGGTACCGTTATTACACAAATGTTCAAATTATTTAT
TTTGTTATTATTTCTACAAATTGTAAAAATATTATATATACACTCCAACTACAATAATAA
ATTTGTTTGACACAAAAATAAAACAAAACCATGTGTAAAATTCGACATATTTATGCATAT
AATAATATTATACAATTTTTTTTCTCTTTTTATCCTTTTATATAGCTTATATTCAATTTT
TAACTAATGGAAATGGTATGCAAATAATTACCATAAATTATTATTTTTATAGACACAAAT
GTATGAATATTGTCTTTCAATAAATTTTTCAAAGTTTCAATTTCTACTTATTTTGTCTAT
CAACATATTACCTTTATACATACATATACTTTTTTTTTACCACATTTTATAGAAGTACAC
AAATTTGCCAACTATCAAAAGATAGGTATAATAGGAAATAATAAAAAATAATATATGCAT
TCAGAATATTTACACATACCAATATGCATCTACTAATATAATTGTTTAATGTGAAAATAA
AAAATTAATTAAATATGTATTCTACTTTTCTGACAATCATCATTGCTCCTCTTAATATAG
AAAGGATCCTCTCTGATGGTAAGGCATATTTTTTTAGCTTTTTTTAGAAAAATAAATATG
ACCATAAGAAAAAGAAAAAAATGAAAAAGATGATGCATTAAGCATTTACATGAAAGTTAT
ATGTTTAATTAAAATGACAATCATAACATCTATGAATCGTATTCTTATAATTTGTTTTCA
ATTTTAAAGACTCATCTAGCAATATTACATCTAAGGTAATATATAAAGATACGTTTTCAT
TAAAAAAATAAATTAGTTCATATTTTTATTTATATATTTAAAAAAACTTTACAAAAAAAG
TAAACAATAATAAAAATATACTAATAAATATACATGTTTAATATTTTTTTAAGGGTTATG
TAGAACCATTTGCATCTGTTAATGAGAAAGGTAAAATCACACATATAAGGATTTACACTC
AAGCTTGCATGCCTGCAGGTCAACAATAAATAATAAATAAATATTGTGGAAATAAAATAA
CATATAATTATTTTTAATACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGATATAA
AAATATATAATAATAACATATATGATTTCAAATTAATCTTTTCAAAAATGGTGTTTATTT
TTGTATGTTTGTGTATGAATTAATCACATAACACATCTATTAAATTGAGTTGGTAATATA
GACACAAATAAATATATATATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTAAGCAT
ATTTTCTTTTTCTTTGGATTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTTTTTGT
TATTTTGTCATTTTGTTATTTTGCTATTTTATGGATTAATTTTTGTTTATAAAATGGGAA
ATAATTTTAACATATTTAAATAAATGGAGAAAAAATATAAAATAATTATAAAAAAAAGTT
AATACACATTTTTTCCTGTTATAGACCTTATATTTATTTATCCATATATATATATATATA
TATATATATATATATATACATACCAAGTGAATTAAGAGGAAAGCTAATTTATTATTCAGA
ATAATATATGAACTATATATAATTTTTATTATTTTGGTGTATATTAATCTGTCTATATGC
ATACATGCAATAATTTATCGACTTATATATCAAATAACATAAAATAGAAGTGTTTTAAAT
TATGGATATATGCTCAATATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTATACAT
TTTATATATGGTCTCTTTTTTTTTTAAATATTATTTAAGTGATCATGAAAATATAAATAA
TTTTTTTTTATTTAATATCCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGTGTTTT
GAARGTTCGGATATAGTTGTATGGACATATAATATATTTTGTGAAAAATTGGTTTTATGT
TTATACTTATGCCAATACTTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATTAAAGC
CAATTTTATAATATATATTTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTTATGGT
AAGTGCTCAAAGAGAGTTGCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTTGAATT
ACACATTAAAAATATATAGATACATATATTATAATATGAAATCATTAATAATTTAGGGAA
ATTCTACAAATTTAAAAACGAATAAAATAATTGTTTTTCATCATGCCATAACACAATATT
GATATATACATGTACAAACATTTTTTTTATTTGGAAAATATAAATTATATAAAAAAAAAT
GTATAGTATACAAAATGAGCATATTCACACGGGGTGGACGTTCATTTTTTCATTTTTCCC
CTGTTTTTTATGAGTATATGATAAAATTTTATGAACATTTACACAAAATGAAAATGGATA
TATAGGAAAAATGGAGCGGTATTTCATTTATCTTTGATTGTCATTTGGATATTATATTAC
CYTGGGTAGGCAATTAAAAATGTTAAATAACAATTTAAGGAAATTATATTTTATATATTA
AAATTAACACTGTATTATATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAAAGCTG
TTTAATATCAATTTTAACAAATTACACACATGTTAATATATTTATATATATAATTTATAT
ATTTATAATTTATATATTTATATTTTTATTATTTATATATTTATTATTTATTGTGTGTGT
CAATTCGGGTAGGATATACCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAAATATA
AAGRATTTGAAAAAGAGAAAGATAGAATATGATCCCATCATATATAGCCCTATAATTTTT
ATTTAGCAGCGAATTAATTTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAATATAT
ATAATACAATAAAAAAGTGCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTTTAAGG
GGTTATATAATATTAAATATATAAAATACGATTATATATTTTTGCTACAATTTTTTATAT
TAAGATATAAATAGTAAATAAATGGTATTATATGGCATGTAATATATAAATTTTTTCCAA
TTTTTATTTTATATACACTTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACACATTC
ATTTTAAAAATTGACTATTTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAGTATTT
TCATTTGTATATTGCTTATATATATAAATACACACCTAAATGTTACAAAGGATCAATGCA
TAAACCGGTGTGTCTGGTCGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAACAACGG
CCTCCCGTGGCCCCACTTGACCACAGATTTCAAACACTTTTCTCGTGTGACAAAAACGAC
GCCCGAAGAAGCCAGTCGCCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACGGGCGA
CTCTGGACTTCCCTCTCCATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGACGGAA
AACCTGGGAAAGCATGCCTCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATCGTCGT
TTCCTCTTCCCTCAAAGAAGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGCCAGCA
GCGCGTCCGAGTCTGTGCTTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAGTACAA
GGATTCTGTCGACCAGATTTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCGCTGTC
TCTGGGCGTTGCCTCTCACCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGCGACGT
TTTCTTCCCTGCGTTCCCCGGAGATGACATTCTTTCAAACAAATCAACTGCTGCGCAGGC
TGCAGCTCCTGCCGAGTCTGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAGAAGGA
CAATGAAGCGACGTATCGACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGGGTTCC
CTACGACTTTGTGGTTCTCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCGGAACC
GAGCAACGCAATGAGCTCCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGGTTGCA
GGCTCCTTCTTCGGCCGCAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAAGACCG
GAAAAAACGCGAGCAAAAGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGAGGCCA
TGAAGAGTTCCAGTACCTTGATCTCATTGCCGACATTATTAACAATGGAAGGACAATGGA
TGACCGAACGGGCGTTGGTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCGCTGGA
TCAGGCCTTTCCACTTCTCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAAGAGTT
GCTGTGGTTCATTCGCGGCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTGAAGAT
CTGGGACAAGAATGTGACACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGAGAGGT
CGGAGACATCGGCCCGGGCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATACAAAGA
CATGCACACAGACTACACAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAGATGCT
GAGAACGAATCCAACAGATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCGCTGGA
CGAAATGGCGCTGCCGCCTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAGAAGGA
GCTGTCGTGCATCATGTATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTCAACAT
CGCTTCCTATTCGCTTTTGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCTAAGGA
GTTCATTCACTTCATGGGGAACACGCATGTCTACACGAACCATGTCGAGGCTTTAAAAGA
GCAGCTGCGGAGAGAACCGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAACGCAT
CAAGGAAATCGACGATTTCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCGCACGG
ACGAATCCAGATGGAGATGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCCCGTTT
TTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTTG
TGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTTT
ATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCATA
TTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGATATTTATGGA
ACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTTTTACTGTTA
TAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTAT
AATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACAC
AAATGATGTTTTTTCCTTCAATTTCGATATCGAATTCAATCAGAAAATGGCATTCTTAAA
TTCCCTACTGAATTTTATGTTAAACGAATAAATATGTATACATACATGCATACATATGTG
TGTGTGTGTAATGGGTTACTACCGCTATCTTTTTATTTCGTTCTGAATTGTCTCATTTTT
ATTTTTGATCTATATTTTCCTATTTTGTTGTTATTTTGAATTTGCTTGGCATATTATTCG
ATCATTAAAAGAGATTAAATGCTTTTCCTACATATATATTAGTATTTTGTCGATTTTATT
AATAATATAAATTTTATCCCGTTTTGTTTTATTTATTTTTTTAAACTTTATTATTATTTT
TTTTTTATTTATTATATTTTTTTTTATGCTATTCGAAAATAATGGCATTTGAGGTTTTGG
AAAAACTACTACACAAAAAAATAAGTTGATAAATTGCCCTTCAAATAAAAAAATATTATA
AATAAATAAATAAATAAAAAAATAAAAATAATCAAATTAAAAATAATAAAATAACGCTAA
ATTGGCTATTTGCTAGCATATCATTTGCTGAATAACTTATTACATATATGTTTTGCATGC
TTGGGATAATTTTTCATTTATATGTATATTATATAAACACAATATAATTACATACACTAT
TTTCGAGTTTTATCTTTATGCTTGTAAAATGTTCTTACTTATTAATATAAGGAAGTATGA
ATCATCTAACGGTTAAAAGTGTAGCCTTATTGGTGGTAGGGATAGGTTATGGAATAATAC
ATTATTACTTAAATTTTAAGAAAAAAAGATTGATAAATTTATTAACAGAAGGGAATATTT
GTATAGTTGTTGCTCATCCCGATGACGAAGTTATGTTTTTTTTACCAACCTTAAAATTGT
TGTTTAATAATAAAAATAAAACTGAAATTTTTATTTTATCCCTTTCTAATGGTAATTATT
ATGGTATCGGAAAAATACGAGAAAAAGAATTTACTAAAGTTTGGTCATATTTAGGGGGCC
ATCCAAATAATTATAAAATAATGGATGATCCAAATATGCAAGATGGTTGGAACCCATGGA
ATGAACGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGC
GCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACT
TAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAC
CGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGG
CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC
CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCC
CCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCT
CGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC
GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAAC
TGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGAT
TTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAA
AATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTT
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAC
CAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC
TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTC
CACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTG
CGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCG
GATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCA
AATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCG
TGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGA
ACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAC
CTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT
CCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC
TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGA
TGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTC
CTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTG
GATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAG
CGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCC
GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGC
AGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACAC
TTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGA
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe genetic modification results in the disruption of the following gene models PB107027.00.0 and PB107193.00.0 and PB001101.03.0 (= MAL6P1.181 = PFF1195c).
See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1AAAGGTACCGTTATTACACAAATGTTC
Additional information primer 1L2648 (Asp718); 5'
Sequence Primer 2AAAAAGCTTGAGTGTAAATCCTTATATG
Additional information primer 2L2649 (HindIII); 5'
Sequence Primer 3AAAGAATTCAATCAGAAAATGGCATTC
Additional information primer 3L2650 (EcoRI); 3'
Sequence Primer 4AAAGCGGCCGCGTTCATTCCATGGGTTCC
Additional information primer 4L2651 (NotI); 3'
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6