Summary

RMgm-105
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_0934600; Gene model (P.falciparum): PF3D7_1113000; Gene product: conserved Plasmodium protein, unknown function
PhenotypeNo phenotype has been described
Last modified: 9 July 2010, 14:47
  *RMgm-105
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 18974882
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA 507cl1 (RMgm-7)
Other information parent lineP.berghei ANKA 507cl1 (RMgm-7) is a reference ANKA mutant line which expresses GFP under control of a constitutive promoter (PubMed: PMID: 16242190).
The mutant parasite was generated by
Name PI/ResearcherE. Lasonder, C.J. Janse, H.G. Stunnenberg
Name Group/DepartmentDepartment of Molecular Biology
Name InstituteNCMLS, Radboud University Nijmegen
CityNijmegen
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-105
Principal name800; 836
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of PBANKA_093460

Protein (function)
Unknown. The protein was detected in a proteome analysis of P. falciparum oocysts and sporozoites. It was highly abundant in oocyst-derived sporozoites and not detected in salivary gland sporozoites. It contains a transmembrane region.

Phenotype
The phenotype has not been analysed in detail and the mutants have not been cloned after transfection. Sporozoite production was not significantly different from wild type and sporozoites were infectious to C57BL/6 mice


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0934600
Gene Model P. falciparum ortholog PF3D7_1113000
Gene productconserved Plasmodium protein, unknown function
Gene product: Alternative name
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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GCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTA
ACCCTCACTAAAGGGAACAAAAGCTGGTACCAATACATTATTAATTGGAATGCAAACTAT
TTTATACTAACTTTTTTAAAATGATTTTATCGAAATTTTTTTAGTATTATATAATTACAT
ATTTATTAACTTTTTTTCATTTTAGACTTATGCTCCTTAATATGTATATTTACACATATA
TGTATGTATGGCTTAGTCATAGTATTGACACAAAAATTGTGAAGGGCAATGTTTAGTTAT
TTGTAAAACATTATTCTTCTCCTTTTTTTTGAATGGTGGTATTGTTTTGTTTCATTACTT
ATTTTTATTAACTTCATATATAGTTATAATTTTGTTCTTTCCTCATATTTATCACATAAA
TTTAAATACACCATTAGAAATATAATTATTTAATTAAAAAACATAAAAATACTTTTAAAA
AAATGTTCTCAAATAATAAAAGAACAATCATTTGTGAGATACTATTTTTTTTCGCTTATT
TTCTAAAAAATGGTAATAAATACTTGCTGTCTTAATAATATTCGATTGATAAATTATGGA
AATATTTTAAGATTTTACGAAAATATATCTCTGTACAATCTACCTAGTGCAATAATCCAT
AACAAATATACATGTAAAGTCATATGCATGTATCGATTAATTCACTCCGTTTTATTGTTT
GCCTTATTTGCCAGGTGGCCAATGTAGCGATATATTTCCAAGGAGTCTGTTTTTAAAAAG
GGTAATAACGAAGAGAAAAGTTGTAATTGAATAAGTTAATATTAGCGCAAAATTAAGAAA
AAGACACTATTATTACAATATAAGATCATCATGTTATGAGCCCATAAATAAAACACAAAT
TATTGTCTTCCTTTAGAACCACCCTTCCAATCTAGTGCATATTTTTAAAGATACCAAAGG
TTAGAGGAACTCAATATATATGTTACTATTTTTTAAATCCTACCTTATTCTTACATCTTA
GTTTTTTTGTATCTACCACGTGGAATACCATTGCTTTGTTCATTTACTTTTCCTATTTAT
TGAAAGTACTAATATTTCAATTGATGCCTTATTTTTCTATCACTACTCGCATATTCTGAT
TATACAAGCTTGCATGCCTGCAGGTCAACAATAAATAATAAATAAATATTGTGGAAATAA
AATAACATATAATTATTTTTAATACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGA
TATAAAAATATATAATAATAACATATATGATTTCAAATTAATCTTTTCAAAAATGGTGTT
TATTTTTGTATGTTTGTGTATGAATTAATCACATAACACATCTATTAAATTGAGTTGGTA
ATATAGACACAAATAAATATATATATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTA
AGCATATTTTCTTTTTCTTTGGATTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTT
TTTGTTATTTTGTCATTTTGTTATTTTGCTATTTTATGGATTAATTTTTGTTTATAAAAT
GGGAAATAATTTTAACATATTTAAATAAATGGAGAAAAAATATAAAATAATTATAAAAAA
AAGTTAATACACATTTTTTCCTGTTATAGACCTTATATTTATTTATCCATATATATATAT
ATATATATATATATATATATATACATACCAAGTGAATTAAGAGGAAAGCTAATTTATTAT
TCAGAATAATATATGAACTATATATAATTTTTATTATTTTGGTGTATATTAATCTGTCTA
TATGCATACATGCAATAATTTATCGACTTATATATCAAATAACATAAAATAGAAGTGTTT
TAAATTATGGATATATGCTCAATATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTA
TACATTTTATATATGGTCTCTTTTTTTTTTAAATATTATTTAAGTGATCATGAAAATATA
AATAATTTTTTTTTATTTAATATCCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGT
GTTTTGAARGTTCGGATATAGTTGTATGGACATATAATATATTTTGTGAAAAATTGGTTT
TATGTTTATACTTATGCCAATACTTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATT
AAAGCCAATTTTATAATATATATTTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTT
ATGGTAAGTGCTCAAAGAGAGTTGCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTT
GAATTACACATTAAAAATATATAGATACATATATTATAATATGAAATCATTAATAATTTA
GGGAAATTCTACAAATTTAAAAACGAATAAAATAATTGTTTTTCATCATGCCATAACACA
ATATTGATATATACATGTACAAACATTTTTTTTATTTGGAAAATATAAATTATATAAAAA
AAAATGTATAGTATACAAAATGAGCATATTCACACGGGGTGGACGTTCATTTTTTCATTT
TTCCCCTGTTTTTTATGAGTATATGATAAAATTTTATGAACATTTACACAAAATGAAAAT
GGATATATAGGAAAAATGGAGCGGTATTTCATTTATCTTTGATTGTCATTTGGATATTAT
ATTACCYTGGGTAGGCAATTAAAAATGTTAAATAACAATTTAAGGAAATTATATTTTATA
TATTAAAATTAACACTGTATTATATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAA
AGCTGTTTAATATCAATTTTAACAAATTACACACATGTTAATATATTTATATATATAATT
TATATATTTATAATTTATATATTTATATTTTTATTATTTATATATTTATTATTTATTGTG
TGTGTCAATTCGGGTAGGATATACCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAA
ATATAAAGRATTTGAAAAAGAGAAAGATAGAATATGATCCCATCATATATAGCCCTATAA
TTTTTATTTAGCAGCGAATTAATTTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAA
TATATATAATACAATAAAAAAGTGCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTT
TAAGGGGTTATATAATATTAAATATATAAAATACGATTATATATTTTTGCTACAATTTTT
TATATTAAGATATAAATAGTAAATAAATGGTATTATATGGCATGTAATATATAAATTTTT
TCCAATTTTTATTTTATATACACTTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACA
CATTCATTTTAAAAATTGACTATTTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAG
TATTTTCATTTGTATATTGCTTATATATATAAATACACACCTAAATGTTACAAAGGATCA
ATGCATAAACCGGTGTGTCTGGTCGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAAC
AACGGCCTCCCGTGGCCCCACTTGACCACAGATTTCAAACACTTTTCTCGTGTGACAAAA
ACGACGCCCGAAGAAGCCAGTCGCCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACG
GGCGACTCTGGACTTCCCTCTCCATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGA
CGGAAAACCTGGGAAAGCATGCCTCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATC
GTCGTTTCCTCTTCCCTCAAAGAAGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGC
CAGCAGCGCGTCCGAGTCTGTGCTTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAG
TACAAGGATTCTGTCGACCAGATTTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCG
CTGTCTCTGGGCGTTGCCTCTCACCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGC
GACGTTTTCTTCCCTGCGTTCCCCGGAGATGACATTCTTTCAAACAAATCAACTGCTGCG
CAGGCTGCAGCTCCTGCCGAGTCTGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAG
AAGGACAATGAAGCGACGTATCGACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGG
GTTCCCTACGACTTTGTGGTTCTCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCG
GAACCGAGCAACGCAATGAGCTCCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGG
TTGCAGGCTCCTTCTTCGGCCGCAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAA
GACCGGAAAAAACGCGAGCAAAAGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGA
GGCCATGAAGAGTTCCAGTACCTTGATCTCATTGCCGACATTATTAACAATGGAAGGACA
ATGGATGACCGAACGGGCGTTGGTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCG
CTGGATCAGGCCTTTCCACTTCTCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAA
GAGTTGCTGTGGTTCATTCGCGGCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTG
AAGATCTGGGACAAGAATGTGACACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGA
GAGGTCGGAGACATCGGCCCGGGCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATAC
AAAGACATGCACACAGACTACACAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAG
ATGCTGAGAACGAATCCAACAGATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCG
CTGGACGAAATGGCGCTGCCGCCTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAG
AAGGAGCTGTCGTGCATCATGTATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTC
AACATCGCTTCCTATTCGCTTTTGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCT
AAGGAGTTCATTCACTTCATGGGGAACACGCATGTCTACACGAACCATGTCGAGGCTTTA
AAAGAGCAGCTGCGGAGAGAACCGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAA
CGCATCAAGGAAATCGACGATTTCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCG
CACGGACGAATCCAGATGGAGATGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCC
CGTTTTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTA
TGTTGTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAAC
ATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAG
CCATATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGATATTT
ATGGAACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTTTTAC
TGTTATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATG
TTTATAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATA
AACACAAATGATGTTTTTTCCTTCAATTTCGATATCGAATTCAAACAGATATTAATAGTA
TTCATAAAATGATTGACGATTTAATAAAACATAAAGATGTTGGATCTCTAAAAACTAGTA
TATTAGAAAATTTGATTGATTTAAATATATTAAATAATAGAAATAATAGGGTTCGAGATA
ATTATTTGAATATATTACATTTAGAAATATTTTCTTTATTTTCTTTTGGGCCATATCATA
ATATATATAATTTATTAGACGAATTATTTTTATATGATAATAAATTATTAAACTGGTTCA
AAAGATGGATAATATTAAATAAGAATACATATTATAATGATAATAATAATTTTAATCAAT
TGCTATTTATATCTTCTATAAAATATATGATAAAAACATTGAGTATGAAAATTAGTAAAA
AAACATTAACAAAAAAATACTTCAAATATATACCTAAATTATACAACAAAAAAAGAGCCA
GAAGTATAGACTTAAGGTTTGAAGAAATTAAACCAAATTCAATTTTAAAAAATGAAATTG
ATTACAAAAAAGAAAATTACGATAGTGATATGGGTAAAATTTTTTTAAATCGTGTCAAAG
AATATAAAATTAGTTCTAAACTTCGAGATAAAATTTTAGAAGAATTAGACAAATATAAAT
TCTCGATATCATTAGACCGTTATAAACAAGATTATTATTTTTTATCTTTAGTAGGTTTAG
GTTATTCATATATTAACAAATTTAGTGAATTAAAGTTAGGCCTATATAAATCTGTTAATA
TTATATTAAAAAAAATGTATAATATATTTTATGTTACTGAATTAATAGAAACTGTAAATG
TATTTGTAAATGTGATTGTAAATATTTATAAAAACATATTTTATAATAATAGTAAATATA
TAATTGAAAATGGAAAAATAAAATTAAGTAAAGAAAAAAAAGAAGAATTAGCTAAATTTA
TCTTATTAGAAGAAAATATAAACATCGATTGTCTACATAATCACATTTACAAAGGGCATA
AGTTTTTTACTCATGATAATTTTGATGATACAAATTATTCAGCGGCCGCCACCGCGGTGG
AGCTCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAA
CGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCT
TTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC
AGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTG
GTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTC
TTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTC
CCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT
GATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAG
TCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCG
GTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAG
CTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTG
GCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAA
ATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGA
AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC
TTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGG
GTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTC
GCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTAT
TATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATG
ACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAG
AATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAA
CGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC
GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCA
CGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTC
TAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTC
TGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTG
GGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTA
TCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAG
GTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGA
TTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATC
TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA
AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA
AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC
CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGT
AGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC
TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC
GATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCA
GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG
CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG
GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGT
TTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAT
GGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT
GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG
CGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCA
GCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGA
GTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGT
GTGGAATTGTGA
Restriction sites to linearize plasmid
Partial or complete disruption of the genePartial
Additional remarks partial/complete disruption See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe genetic modification results in the disruption of the following gene models PB001073.00.0, PB000529.02.0 and PB000863.00.0 (= PF11_0528).
See for more information about the disruption Lasonder, E. et al., 2008, PloS Pathogens 10, e10000195 (Supporting Information: S3).
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1AAAGGTACCAATACATTATTAATTGG
Additional information primer 1L2644 (Asp718); 5'
Sequence Primer 2AAAAAGCTTGTATAATCAGAATATGCG
Additional information primer 2L2645 (HindIII); 5'
Sequence Primer 3AAAGAATTCAAACAGATATTAATAG
Additional information primer 3L2646 (EcoRI); 3'
Sequence Primer 4AAAGCGGCCGCTGAATAATTTGTATCATC
Additional information primer 4L2647 (NotI); 3'
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6